ABSTRACT
Objective@#To make a postural transfer belt for patients with cervical spinal cord injury, to reduce and prevent the corresponding nursing problems with traditional methods of transfer@*Methods@#44 patients with cervical spinal cord injury admitted to the ward from January to June 2017 were selected as the control group by traditional methods of postural metastasis, and 48 patients with cervical spinal cord injury admitted to the ward from July to December 2017 were selected as the observation group by using self-made transfer belt.@*Results@#Number of the skin injury caused by transfer in observation group was 0 cases, in control group was four cases, and there was significant difference (χ2=1.91, P=0.049). Falls occur due to metastasis: there were 0 cases in the observation group and five cases in the control group, there was significant difference (χ2=2.39, P=0.026). The number of caregivers in the observation group decreased on average (1.21±0.08), and the number of caregivers in the control group decreased on average (0.86±0.09). The differences between the two groups are statistically significant (t=2.905, P=0.005). The results of the investigation on the escorts in the observation group showed that 46 (95.8%) people believed that the transfer belt was convenient for patients. 47 people believed that the transfer belt was safer and more labor-saving to use, accounting for 97.9%, and 47 people believed that the transfer belt would continue to be used when they went home, who occupied 97.9%.@*Conclusion@#The self-made transfer belt is convenient for the caregivers to carry the patients with cervical spinal cord injury, which effectively prevents the occurrence of skin injury, falls and other nursing problems, and improves the quality and experience of the caregivers. This technology is worthwhile to spread.
ABSTRACT
Background: Rice seed proteins are lacking essential amino acids [EAAs]. Genetic engineering offers a fast and sustainable method to solve this problem as it allows the specific expression of heterologous EAA-rich proteins. The use of selectable marker gene is essential for generation of transgenic crops, but might also lead to potential environmental and food safety problems. Therefore, the production of marker-free transgenic crops is becoming an extremely attractive alternative and could contribute to the public acceptance of transgenic crops
Objectives: The present study was conducted to examine whether AmA1 can be expressed specifically in rice seeds, and generate marker-free transgenic rice with improved nutritive value
Materials and Methods: AmA1 was transferred into rice using Agrobacterium-mediated co-transformation system with a twin T-DNA binary vector and its integration in rice genome was confirmed by southern blot. Transcription of AmA1 was analyzed by Real-Time PCR and its expression was verified by western analysis. Protein and amino acid content were measured by the Kjeldahl method and the high-speed amino acid analyzer, respectively
Results: Five selectable marker-free homozygous transgenic lines were obtained from the progeny. The expression of recombinant AmA1 was confirmed by the observation of a 35 kDa band in SDS-PAGE and western blot. Compared to the wild-type control, the total protein contents in the seeds of five homozygous lines were increased by 1.06 tilde 12.87%. In addition, the content of several EAAs, including lysine, threonine, and valine was increased significantly in the best expressing line
Conclusions: The results indicated that the amino acid composition of rice grain could be improved by seed-specific expression of AmA1
Subject(s)
Amaranthus , Agrobacterium , Amino Acids, Essential , Genes, PlantABSTRACT
Objective In order to know nurses'congnition of reporting adverse events and errors voluntarily by questionnaire,and then analyze the related factors. Methods Investigated 275 nurses from 25 hospitals in Guangxi Province by self-desinged questionnaire to know their cognition about reporting adverse events and errors voluntarily,analyzed the datum of invetigation. Results There was different ideas about reporting adverse events and errors voluntarily in nurses with different professional title and duty.Nurses'attitude was different under the different system of reporting adverse events and errors. Conclusions The safety awarness of nurses should be strengthened.The existing reporting system must be imported. It is necessary to establish a comprehensive reporting system of care mistakes and adverse events.